A SENSITIVE METHOD FOR MEASURING PYRIMIDINE DIMERS IN SITU |
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| Authors: | J J Cornelis J Rommelaere † J Urbain ‡ M Errera |
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| Institution: | Département de Biologie Moléculaire, UniversitéLibre de Bruxelles, rue des Chevaux, 67 1640 Rhode St Genèse, Belgium |
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| Abstract: | Abstract. –An immunocytological method for detecting pyrimidine dimers in situ has been developed. The technique is based on the fixation in cell nuclei of radiolabelled antibodies directed against ultraviolet irradiated DNA. The relative amount of bound antibodies was estimated by radioautography. Pyrimidine dimers induced by a dose as low as 2 J m-2 can easily be detected. With this technique, the dose response of DNA photoproducts and their elimination by excision and photoreactivation were followed in eukaryotic cell cultures. |
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