高效液相色谱法测定柳树皮提取物中的水杨甙

 建立了一种测定柳树皮提取物中水杨甙的反相高效液相色谱法。该法采用KromasilC18(4 6mmi.d .× 2 5 0mm ,5 μm)色谱柱 ,以甲醇 0 0 1mol/LKH2 PO4 缓冲液 (pH 4 0 1) (体积比为 15∶85 )的混合溶液作流动相 ,流速为1 0mL/min ,紫外检测波长为 2 6 5nm ,灵敏度为 0 0 4AUFS。在 8 89mg/L～ 2 84 40mg/L的范围内 ,水杨甙的峰面积Y与其质量浓度X的线性关系良好 ,回归方程为Y =- 2 91 5 2 6 0 +5 0 0 0 0 34X(r =0 9998)。水杨甙的平均回收率为 96 1%～ 10 1 2 % (n =5 ) ,相对标准偏差 (RSD)为 1 43%。该法操作简便、快速、准确。

Determination of Salicin in Extract of Willow Bark by High Performance Liquid Chromatography

An HPLC method for the determination of salicin in extract of willow bark is described. Chromatographic analysis was carried out on a Kromasil C18, 5 microns column(4.6 mm i.d. x 250 mm) with methanol-0.01 mol/L KH2PO4 buffer (pH 4.01) (15:85, volume ratio) as mobile phase. The detection wavelength was 265 nm. Salicin was extracted from samples with methanol-water(50:50, volume ratio), and centrifuged. Ten microL of supernatant were injected. The average recoveries were from 96.1% to 101.2% (n = 5), and the relative standard deviation (RSD) was 1.43%. The method is simple, rapid and accurate.