Direct electrochemistry of cytochrome c on nanotextured diamond surface |
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Authors: | Nianjun Yang Rene Hoffmann Waldemar Smirnov Armin Kriele Christoph E. Nebel |
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Affiliation: | Fraunhofer-Institute for Applied Solid State Physics (IAF), Freiburg 79108, Germany |
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Abstract: | Nanotextured diamond surfaces with geometrical properties close to protein dimensions were used for the realization of direct electron transfer of cytochrome c (cyt c) without any covalent bonding. The peroxidase activity of native and denatured cyt c was also investigated. Cyclic voltammograms of native cyt c show quasi-reversible electron transfer reactions, while no heme redox activity is detected for denatured cyt c. Unfolding (denaturation) of cyt c can be achieved in the presence of hydrogen peroxide. Partially or fully denatured cyt c showed higher peroxidase activity than native cyt c. This is because denatured cyt c loses its tertiary structure and hydrogen peroxide is easier to access the heme redox center. The apparent Michaelis–Menten constant Km for native and denatured cyt c has been determined to be 0.23 mM and 0.08 mM. |
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