Investigation on interaction of prulifloxacin with pepsin: A spectroscopic analysis |
| |
Authors: | Yabei Huang Jie Yan Benzhi Liu Zhang Yu Xiaoyan Gao Yingcai Tang Yanqin Zi |
| |
Institution: | 1. Department of Chemistry, Huaibei Coal Normal College, No. 100 Dongshan Road, Huaibei, Anhui Province 235000, China;2. Huaibei Occupational Disease Prevention and Cure Hospital, No. 1 Xiangshan Road, Huaibei, Anhui Province 235000, China |
| |
Abstract: | The interaction between prulifloxacin, a kind of new oral taking antibiotic and pepsin, a kind of enzyme in the stomach has been investigated in vitro under a simulated physiological condition by different spectroscopic methods. The intrinsic fluorescence of pepsin was strongly quenched by prulifloxacin. This effect was rationalized in terms of a static quenching procedure. The binding parameters have been evaluated by fluorescence quenching methods. The negative value of ΔG0 reveals that the binding process is a spontaneous process. The binding distance R between donor (pepsin) and acceptor (prulifloxacin) was obtained according to the Förster's resonance energy transfer theory and found to be 0.95 nm. The results obtained herein will be of biological significance in pharmacology and clinical medicine. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|