Real-time monitoring of induced adaptation of redox active Escherichia coli biofilm by EQCM-controlled extracellular redox environment |
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Authors: | Xuan-Hui Xie Erin Lin Li Zi Kang Tang |
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Affiliation: | Department of Physics and William Mong Institute of Nano Science and Technology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong |
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Abstract: | In this paper we report the anaerobic Escherichia coli biofilm formation on solid substrate under redox-controlled extracellular environment by an electrochemical quartz crystal microbalance (EQCM) method. Both biomass and electrochemical activity were monitored in situ. Larger biomass was yielded under redox-controlled condition comparing with natural biofilm growth, which was also confirmed by optical observation. Surface-colonizing cells responded more sensitively to their redox environment than planktonic cells. Cyclic voltammogram (CV) obtained during the time course of biofilm development indicates the emergence of redox active phenotype under redox-controlled condition but not in natural condition. Our results suggest an effective means to control biofilm development with desired metabolic adaptation and also to in situ monitor the biomass yield and the emergent catalytic property simultaneously. |
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