和田维吾尔族老人5-HTTLPR基因插入/缺失多态性研究及长寿的关联分析

 本文运用聚合酶链式反应技术(PCR)对新疆和田地区30例90岁以上维吾尔族长寿老人和70例当地健康、无血缘关系、生活习惯相似、随机抽样的维吾尔族对照人群进行5-HTTLPR基因分型及相应等位基因频率的比较并且探讨了5-羟色胺转运体基因连锁多态性区域(5-HTTLPR)的44bp插入/缺失序列多态性与长寿的相关性。结果表明,维吾尔族长寿老人的5-HTTLPR基因多态性的LL(528bp/528bp)基因型和L(528bp)等位基因频率分别为70%和81.7%,对照者LL基因型和L等位基因频率分别为40％和57.2％,两组之间比较差异有显著性(P<0.05)。由此得出,5-HTTLPR基因多态性与新疆和田地区维吾尔族老人的长寿有关联,新疆和田地区长寿相关基因组成为LL基因型和L等位基因。

The Polymorphism and Its Longevity Correlation Analysis of 5-HTTLPR Gene Insertion/Deletion for the Hotan Uyghur Elderly

In this paper, the polymerase chain reaction (PCR) technology is used to study 30 cases of the Uyghur elderly of over 90 years old in Hotan Prefecture and 70 cases of local health Uyghur people, genetically unrelated, with similar-habits, and random-sampled, as controls to compare the frequencies of the 5-HTTLPR genotypes and the corresponding alleles and to explore the relatiionship between the 44bp insertion/deletion polymorphism of the 5-hydroxytryptamine transporter gene linked polymorphism region (5-HTTLPR) with longevity. The results show that with respect to the 5-HTTLPR gene polymorphism among the Uyghur elderly, the frequencies of the LL (the 528bp/528bp) genotype and L (528bp) allele are 70% and 81.7%, respectively; while among the controls, they are, respectively, 40% and 57.2%, and the difference between the two groups is significant (P<0.05). It is shown that the 5-HTTLPR gene polymorphism and the longevity of Xinjiang Hotan Uyghur elderly are correlated, and, Xinjiang Hotan longevity-correlated gene consists of the LL genotype and the L allele.