维拉帕米作用下急性心梗大鼠比较蛋白质组学研究

以急性心梗大鼠为研究对象, 应用双向凝胶电泳法(2-DE)分析比较了维拉帕米作用下急性心梗大鼠心肌蛋白表达的差异, 从蛋白质水平探讨了维拉帕米心肌保护作用的发生机制. 结果表明, 与假手术组及模型组相比, 维拉帕米给药组心肌组织中有8个蛋白点表达显著上调, 7个蛋白点表达显著下调. 采用质谱(MALDI-TOF-MS)分析结合数据库检索, 共鉴定了其中的15种蛋白质, 可按功能分为如下4类: (1) 能量代谢及线粒体功能相关蛋白; (2) 氧化应激相关蛋白; (3) 细胞骨架蛋白; (4) 其它蛋白. 研究结果表明, 维拉帕米的心肌保护作用与恢复心肌损伤过程中的能量供应及对抗氧化应激等作用有关.

Proteome Study of Differential Protein Expression in Acute Infracted Rat Heart after Verapamil Treatment

To explore the protein-level action mechanism of verapamil in acute myocardial infracted rats,the myocardial proteome was analyzed by two-dimensional electrophoresis(2-DE).In comparison with the sham-operated group and the infracted group,the result shows that eight proteins expressions in verapamil treated group were up-regulated,and seven proteins expression in this group were down-regulated significantly.By using MALDI-TOF-MS,15 proteins with significant changes were identified by database searching.These proteins can be divided into four groups via their biological function:(1) energy metabolism and mitochondrial function related proteins;(2) oxidative stress-induced proteins;(3) cytoskeletal proteins;(4) other proteins.The findings show that the myocardial protective effects of verapamil in myocardial damage process were related to the recovery of energy supply as well as anti-oxidative stress property.