Vitamin C-silver: an environmentally benign choice for DNA visualization on polyacrylamide gels |
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| Authors: | Zhu Zhongxin Cong Weitao He Hongzhang Wang Xi Chen Mao Hong Guoying Jin Litai |
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| Affiliation: | Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College, Wenzhou, P. R. China. |
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| Abstract: | A practical, sensitive and environment-benign protocol for the detection of DNA on polyacrylamide gels was described. In this method, the most commonly used formaldehyde-based developer in DNA silver stain, which poses potential hazards to the health of operators, is firstly replaced by vitamin C (Vc) in sodium thiosulfate solution. This allows user-friendly and efficient visualization of DNA that takes about 20 min to complete all the procedures, and provides comparable sensitivity (8 pg of single band) to the most sensitive formaldehyde-based silver staining method developed before. |
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