Comparison of different immunoaffinity clean-up procedures for high-performance liquid chromatographic analysis of ochratoxin A in wines |
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Authors: | Castellari M Fabbri S Fabiani A Amati A Galassi S |
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Institution: | Università degli Studi di Bologna, Corso di Laurea in Scienze e Tecnologie Alimentari, Cesena (FC), Italy. cast@foodsci.unibo.it |
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Abstract: | Three immunoaffinity clean-up procedures to analyse ochratoxin A (OTA) in wines were compared. The direct wine clean-up with Ochraprep and OchraTest columns gave equivalent results in terms of recovery and precision if compared with the reference procedure involving a preliminary extraction of OTA with chloroform. OTA quantification limit in wine ranged from 0.020 to 0.045 microg/l. The 'on-flow' OTA emission spectrum (excitation 333 nm) showed a maximum at 460 nm and could be used to confirm the quantitative results. The analysis of 11 red and white wines gave no significant quantitative differences between the three clean-up techniques. |
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