Single molecule acid-base kinetics and thermodynamics |
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Authors: | Mason Michael D Ray Krishanu Grober Robert D Pohlers Gerd Cameron James |
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Institution: | Department of Applied Physics, Yale University, New Haven, Connecticut 06520, USA. |
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Abstract: | We report a method in which temperature dependent single-molecule fluorescence measurements are used to study the kinetics and thermodynamics of the acid-base interaction in films of photoresist polymer. We use the two distinct fluorescent prototropic forms of Coumarin 6 (C6-->C6+) to indicate the state of the acid-base system. Data are analyzed using a statistical model of the intensity probability distributions, yielding temperature dependent proton exchange rates, which is confirmed through agreement with a simple two-state Monte Carlo model. The temperature dependent rates are used to calculate the activation enthalpy for proton exchange. |
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