New solid support for the synthesis of 3'-oligonucleotide conjugates through glyoxylic oxime bond formation |
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Authors: | Spinelli Nicolas Edupuganti Om Prakash Defrancq Eric Dumy Pascal |
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Institution: | Département de Chimie Moléculaire, UMR CNRS 5250, ICMG FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France. |
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Abstract: | A novel solid support 1 was synthesized to incorporate glyoxylic aldehyde functionality at the oligonucleotide 3'-terminus. 6-mer and 11-mer oligonucleotide sequences containing 3'-glyoxylic aldehyde functionality were prepared by using this support. These modified oligonucleotides were coupled to reporters containing an aminooxy group to prepare oligonucleotide 3'-conjugates through glyoxylic oxime bond formation. The hydrolytic stability of a glyoxylic oxime linkage was also investigated. reaction: see text]. |
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