A selective Seoul-Fluor-based bioprobe, SfBP, for vaccinia H1-related phosphatase--a dual-specific protein tyrosine phosphatase |
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Authors: | Jeong Myeong Seon Kim Eunha Kang Hyo Jin Choi Eun Joung Cho Alvin R Chung Sang J Park Seung Bum |
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Institution: | BioNanotechnology Research Center, KRIBB and NanoBio Major, UST, 111 Kwahangno, Yuseong, Daejeon 305-806, Korea. |
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Abstract: | We report a Seoul-Fluor-based bioprobe, SfBP, for selective monitoring of protein tyrosine phosphatases (PTPs). A rational design based on the structures at the active site of dual-specific PTPs can enable SfBP to selectively monitor the activity of these PTPs with a 93-fold change in brightness. Moreover, screening results of SfBP against 30 classical PTPs and 35 dual-specific PTPs show that it is selective toward vaccinia H1-related (VHR) phosphatase, a dual-specific PTP (DUSP-3). |
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