Enhanced Aryltetralin Lignans Production in Linum Adventi-Tious Root Cultures |
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| Authors: | Michela Alfieri,Iride Mascheretti,Romé o A. Dougué Kentsop,Roberto Consonni,Franca Locatelli,Monica Mattana,Gianluca Ottolina |
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| Affiliation: | 1.Institute of Chemical Sciences and Technologies “Giulio Natta”, National Research Council, Via Mario Bianco 9, 20131 Milan, Italy; (M.A.); (R.C.);2.Institute of Agricultural Biology and Biotechnology, National Research Council, Via Bassini 15, 20133 Milan, Italy; (I.M.); (R.A.D.K.); (F.L.); (M.M.) |
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| Abstract: | Lignans are the main secondary metabolites synthetized by Linum species as plant defense molecules. They are also valuable for human health, in particular, for their potent antiviral and antineoplastic properties. In this study, the adventitious root cultures of three Linum species (L. flavum, L. mucronatum and L. dolomiticum) were developed to produce aryltetralin lignans. The effect of two elicitors, methyl jasmonate and coronatine, on aryltetralin lignans production was also evaluated. The adventitious root cultures from L. dolomiticum were obtained and analyzed for the first time and resulted as the best producer for all the aryltetralins highlighted in this system: Podophyllotoxin, 6-methoxypodophyllotoxin and 6-methoxypodophyllotoxin-7-O-β-glucoside, the last showing a productivity of 92.6 mg/g DW. The two elicitors differently affected the production of the 6-methoxypodophyllotoxin and 6-methoxypodophyllotoxin-7-O-β-glucoside. |
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| Keywords: | Linum aryltetralin lignans 6-methoxypodophyllotoxin elicitor NMR methyl jasmonate coronatine |
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