Recent Advances about the Applications of Click Reaction in Chemical Proteomics |
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| Authors: | Tingting Yao Xiaowei Xu Rong Huang |
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| Affiliation: | 1.School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, China;2.State Key Laboratory of Natural Medicines, Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China |
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| Abstract: | Despite significant advances in biological and analytical approaches, a comprehensive portrait of the proteome and its dynamic interactions and modifications remains a challenging goal. Chemical proteomics is a growing area of chemical biology that seeks to design small molecule probes to elucidate protein composition, distribution, and relevant physiological and pharmacological functions. Click chemistry focuses on the development of new combinatorial chemical methods for carbon heteroatom bond (C-X-C) synthesis, which have been utilized extensively in the field of chemical proteomics. Click reactions have various advantages including high yield, harmless by-products, and simple reaction conditions, upon which the molecular diversity can be easily and effectively obtained. This paper reviews the application of click chemistry in proteomics from four aspects: (1) activity-based protein profiling, (2) enzyme-inhibitors screening, (3) protein labeling and modifications, and (4) hybrid monolithic column in proteomic analysis. |
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| Keywords: | click reaction chemical proteomics activity-based protein profiling hybrid monolithic column |
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