Bioassay-Guided Isolation of Two Eudesmane Sesquiterpenes from Lindera strychnifolia Using Centrifugal Partition Chromatography |
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| Authors: | Ji Hoon Kim Je-Seung Jeon Jung Hoon Kim Eun Ju Jung Yun Jung Lee En Mei Gao Ahmed Shah Syed Rak Ho Son Chul Young Kim |
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| Institution: | 1.College of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan 15588, Korea; (J.H.K.); (J.-S.J.); (J.H.K.); (E.J.J.); (Y.J.L.); (E.M.G.); (R.H.S.);2.Department of Pharmacognosy, Faculty of Pharmacy, University of Sindh, Jamshoro 76080, Pakistan;3.R&D Center, Huons Co., Ltd., Ansan 15588, Korea |
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| Abstract: | In this study, a centrifugal partition chromatography (CPC) separation was applied to identify antioxidant-responsive element (ARE) induction molecules from the crude extract of Lindera strychnifolia roots. CPC was operated with a two-phase solvent system composed of n-hexane-methanol-water (10:8.5:1.5, v/v/v) in dual mode (descending to ascending), which provided a high recovery rate (>95.5%) with high resolution. Then, ARE induction activity of obtained CPC fractions was examined in ARE-transfected HepG2 cells according to the weight ratios of the obtained fractions. The fraction exhibiting ARE-inducing activity was further purified by preparative HPLC that led to isolation of two eudesmane type sesquiterpenes as active compounds. The chemical structures were elucidated as linderolide U (1) and a new sesquiterpene named as linderolide V (2) by spectroscopic data. Further bioactivity test demonstrated that compounds 1 and 2 enhanced ARE activity by 22.4-fold and 7.6-fold, respectively, at 100 μM concentration while 5 μM of sulforaphane induced ARE activity 24.8-fold compared to the control. |
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| Keywords: | Lindera strychnifolia centrifugal partition chromatography linderolide V eudesmane sesquiterpenes |
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