Capillary electrophoresis of anthraquinones from Cassia siamea |
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Authors: | Koyama Junko Morita Izumi Tagahara Kiyoshi Bakari Jameelah Aqil Mohammad |
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Institution: | Kobe Pharmaceutical University, Kobe, Japan. j-koyama@kobepharma-u.ac.jp |
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Abstract: | The separation and determination of two anthraquinones, emodin and chrysophanol, and two bianthraquinones, cassiamin A and cassiamin B, were achieved by capillary electrophoresis (CE). The running electrolyte used in this method was 0.05 M hydroxypropyl-gamma-cyclodextrin in 0.1 M borate buffer (pH 9) containing 10% acetonitrile, with an applied voltage of 20 kV. Application of this technique in the determination of the main bianthraquinones, cassiamin A and cassiamin B, of Cassia siamea is demonstrated in this paper. |
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