Semi-Constant-Time HMSQC (SCT-HMSQC-HA) for the Measurement of JHH Couplings in N-Labeled Proteins |
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Authors: | Helena Aitio Perttu Permi |
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Institution: | NMR Laboratory, Institute of Biotechnology, P.O. Box 56, FIN-00014, University of Helsinki, Finland |
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Abstract: | A simple method for accurately measuring 3JHNHα coupling constants in 15N-labeled proteins is described. This semi-constant-time HMSQC-HA experiment combines the rapidity and convenience of the recently introduced CT-HMQC-HA scheme (Postingl and Otting, J. Biomol. NMR 12, 319–324 (1998)) with the high resolution and robustness of the HSQC experiment. The proposed method is demonstrated for the 76-residue human ubiquitin and Saccharopolyspora erythraea calerythrin (176 residues). Our results imply that the SCT-HMSQC-HA experiment is suitable also for proteins with less favorable NMR properties due to its good resolution and sensitivity. |
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Keywords: | coupling constant HSQC CT-HMQC-HA SCT-HMSQC-HA NMR |
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