Identification and Fibrinolytic Evaluation of an Isoindolone Derivative Isolated from a Rare Marine Fungus Stachybotrys longispora FG216 |
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| Authors: | Ge Wang Wenhui Wu Quangang Zhu Shiqing Fu Xiaoyu Wang Shaotong Hong Ruihua Guo Bin Bao |
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| Institution: | 1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;2. Institute of Marine Science, Shanghai Ocean University, Shanghai 201306, China;3. Department of Chinese Pharmacy, Yueyang Hospital of Integrated Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China;4. Shanghai Engineering Research Center of Aquatic‐Product Processing and Preservation, Shanghai 201306, China |
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| Abstract: | An isoindolone derivative, Fungi fibrinolytic compound (R)‐2,5‐bis((2R,3R)‐2‐((E)‐4,8‐dimethylnona‐3,7‐dien‐1‐yl)‐3,5‐dihydroxy‐2‐methyl‐7‐oxo‐3,4,7,9‐tetrahydropyrano2,3‐e]isoindol‐8(2H)‐yl)pentanoic acid (FGFC1, Fungi fibrinolytic compound 1 ), was isolated from a rare marine microorganism strain Stachybotrys longispora FG216. The structure of FGFC1 was elucidated by 1H NMR, 13C NMR, IR, and MS data; moreover, it was also evaluated for fibrinolytic activity in vitro and in vivo. The results showed that 0.1–0.4 mmol/L of FGFC1 could stimulate generation of plasmin activity (increased by 2.05–11.44 folds) by measuring Glu‐plasminogen and Lys‐plasminogen activation in vitro. The experiment of fluorescein isothiocyanate (FITC)‐fibrinogen degradation indicated that the effect of FGFC1 on fibrinolytic activity was mediated by plasminogen and scuPA. In addition, FGFC1 (10 mg/kg) could dissolve most of pulmonary thrombus of Wistar rat in vivo. It is possible that FGFC1 is a potential thrombolytic agent in the future. |
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| Keywords: | Stachybotrys longispora FG216 marine microorganism plasmin activity fibrinolytic activity |
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