Column purification and HPLC determination of teicoplanin and A40926 |
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Authors: | E. Riva M. Zanol E. Selva A. Borghi |
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Affiliation: | (1) Merrell Dow Research Institute, Lepetit Research Center, Via R. Lepetit 34, 21040 Gerenzano, VA, Italy |
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Abstract: | Summary A simple HPLC method for quali- and quantitative determination of teicoplanin and A40926 in fermentation broths and in biological fluids is described. The sample is purified on affinity chromatography and then analyzed on a reverse phase column using a gradient phosphate buffer-acetonitrile as mobile phase and UV detection at 254 nm. No interference from endogenous sources has been observed. The analytical method, with partial modifications, was also used to isolate on semipreparative scale the single components of these antibiotics, in order to investigate their structure and their biological activity. |
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Keywords: | Affinity chromatography Column liquid chromatography Glycopeptide antibiotics Purification and determination |
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