Analysis of the fusariotoxins zearalenone and vomitoxin (deoxynivalenol) in human foods and animal feeds by high-performance liquid chromatography (HPLC) |
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Authors: | H Schweighardt J Böhm A M Abdelhamid J Leibetseder M Schuh E Glawischnig |
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Institution: | (1) Institut für Ernährung, Veterinärmedizinische Universität Wien, Linke Bahngasse 11, 1030 Wien, Austria;(2) II. Medizinische Universitätsklinik für Klauentiere, Veterinärmedizinische Universität Wien, Linke Bahngasse 11, 1030 Wien, Austria;(3) Institute of Animal Production, Faculty of Agriculture, Univ. of Mansoura, Egypt |
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Abstract: | Summary HPLC procedures for analyses of the fusariotoxins zearalenone and vomitoxin in individual food- and feedstuffs as well as in mixed feed are described. Zearalenone is separated on a column with polar stationary phase (25 cm × 4.6 mm i.d., particle size 7 m), eluted with a chloroform-isooctane (75/25, v/v)+1.5% methanol mixture and detected fluorometrically. The quantitative determination was possible in all analyzed samples with a detection limit of 2 g/kg with 70–80% recovery. Vomitoxin is fractionated by HPLC (C
18
1
column, 25 cm×4 mm i.d., 5 m particle size) with water-methanol (60/40, v/v) mobile phase and determined by combining GLC or TLC with UV detection. The detection limit in mixed feed with interfering substances was 25 g/kg (recovery 25–35%). The separation by HPLC makes preparation of pure vomitoxin possible. The described methods are fast, simple and low cost and are suitable for routine analyses. |
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Keywords: | HPLC methods Mycotoxins Zearlenone Vomitoxin (Deoxynivalenol) |
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