| 双氧水活化-面粉酶抑制法测定蔬菜中有机磷农药 |
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| 引用本文： | 朱霞萍,何秋玲,尹继先,汪模辉,王以明,戴大华.双氧水活化-面粉酶抑制法测定蔬菜中有机磷农药[J].广州化学,2006,31(3):32-36. |
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| 作者姓名： | 朱霞萍 何秋玲 尹继先 汪模辉 王以明 戴大华 |
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| 作者单位： | 1. 成都理工大学,材料与化学化工学院,四川,成都,610059
2. 广东增城出入境检验检疫局,广东,广州,511340 |
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| 摘 要： | 基于α-乙酸萘酯和固蓝B在面粉酶的催化作用下生成紫红色的偶氮化合物,而有机磷农药在双氧水的活化下会抑制酶的催化作用,其抑制程度与农药的浓度在一定范围内呈线性关系,由此建立了一种快速测定有机磷农药的方法。探讨了最佳的反应条件,测定甲基对硫磷和甲胺磷的线性范围分别为1~10μg/mL,1~50μg/mL,最低检出限分别为0.1136μg/mL,0.3525μg/mL。该法具有试剂廉价易得、操作简便、迅速的特点,适合果蔬中常见有机磷农药的快速检测。
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| 关 键 词： | 有机磷农药 面粉酶 蔬菜 残留分析 |
| 文章编号： | 1009-220X(2006)03-0032-05 |
| 修稿时间： | 2005年12月7日 |
Determination of Organo-Phosphorous Pesticides in Vegetable by The Method of Activation of Hydrogen Peroxide Solution and Inhibition of Wheat Enzyme |
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| ZHU Xia-ping,HE Qiu-ling,YIN Ji-xian,WANG Mo-hui,Wang Yi-ming,Dai Da-hua.Determination of Organo-Phosphorous Pesticides in Vegetable by The Method of Activation of Hydrogen Peroxide Solution and Inhibition of Wheat Enzyme[J].Guangzhou Chemistry,2006,31(3):32-36. |
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| Authors: | ZHU Xia-ping HE Qiu-ling YIN Ji-xian WANG Mo-hui Wang Yi-ming Dai Da-hua |
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| Abstract: | A new method for the determination of organo-phosphorous pesticide has been studied based on its inhibitory action to the wheat enzyme, which can catalyze the reaction of a-naphthyl acetate and diazo fast blue. The optimum condition of the reaction has been investigated. The linear range of determination of methyl-parathion and methamidophos is 1 ~ 10 μg/mL and 1 ~ 50 μg/mL, respectively. The detection limit is 0.1136 μg/mL and 0.3525 μg/mL, respectively. The method has the advantages of being rapid effective and cheap compared to instrumental analytical and other detection methods, and can be applied to pesticide residue analysis in fruit and vegetable. |
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| Keywords: | organo-phosphorous pesticide wheat enzyme vegetable pesticide residue analysis |
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