Real-time imaging of radioisotope labeled compounds in a living plant |
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| Authors: | S. Kanno H. Rai T. Ohya Y. Hayashi K. Tanoi T. M. Nakanishi |
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| Affiliation: | (1) Laboratory of Radioisotope Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;(2) CREST, JST, Japan |
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| Abstract: | We developed a quantitative, real-time imaging system of labeled compounds in a living plant. The system was composed of CsI scintillator to convert β-rays to visible light and an image intensifier unit (composed of GaAsP semiconductor and MCP; micro channel plate) to detect extremely weak light. When the sensitivity and resolution of the image of our system was compared with that of an imaging plate (IP), the sensitivity of our system (with 20 minutes) was higher than that of an IP, with similar quality to that of an IP. Using this system, the translocation of 32P in a soybean plant tissue was shown in successive images. Shared first authors. |
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