微乳液和微乳液凝胶中脂肪酶催化的酯合成反应

在ACT/异辛烷/水形成的油包水微乳液中，研究了Candidalipolytical(CL)脂肪酶催化庚酸和庚醇的酯化反应，动力学研究表明反应符合乒乓(Ping-Pong)BiBi机制，两底物酸和醇均有抑制效应，并测定了反应的表观动力学常数，将CL脂肪酶固定于含明胶的微乳液凝胶(MBGs)中，可制得固定化脂肪酶，含酶的MBGs在非极性有机溶剂中可作为一种新的固相催化剂，并研究了MBGs在异辛烷中催化合成酯反应的性能，所制得的MBGs重复利用性和贮存稳定性都非常好。

Esterification reaction catalyzed by lipase in W/O microemulsion based organogels

Esterification reactions of heptanoic acid with heptanol, catalyzed by Candida lypolytical (CL) lipase, was studied in water-in-oil microemulsions formed by water/bis-(2-ethylhexyl) sulfosuccinate sodium (AOT)/isooctane. Kinetic studies showed that the reaction follows a Ping-Pong Bi Bi mechanism with inhibition by both substrates. The values of all apparent kinetic parameters were determined. Lipase has also been immobilized in gelatin-containing AOT microemulsion-based organogels (MBGs) without any loss of catalytic activity. These lipase-containing MBGs prove to be novel solid-phase catalysts for use in apolar organic solvents and have good storage stability. These lipase-containing MBGs retained its higher activity after many runs esterification reactions.