A rapid and specific approach for direct measurement of donepezil concentration in human plasma by LC-MS/MS employing solid-phase extraction |
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Authors: | Shah Hiten J Kundlik Mohan L Pandya Ankit Prajapati Shivkumar Subbaiah Gunta Patel Chhagan N Patel Dasharath M Suhagia Bhanu N Suhagiya Bhanu N |
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Institution: | Bioanalytical Laboratory, Torrent Research Centre, Bhat, Gandhinagar-382428, Gujarat, India. hitenshah@torrentpharma.com |
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Abstract: | A selective, rapid and simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method is described for assay of donepezil in human plasma using escitalopram as an internal standard. Chromatographic separation was achieved on a Betabasic-C(8), 5 microm, 100 x 4.6 mm column using methanol:water:formic acid (90:9.97:0.03, v/v/v) as mobile phase. Detection of donepezil and internal standard was achieved by ESI MS/MS in positive ion mode using 380.20/91.10 and 325.13/262.00 transitions, respectively. The linearity over the concentration range of 0.15-50 ng/mL for donepezil was obtained and the lower limit of quantification was 0.15 ng/mL. For each level of quality control samples, inter-day and intra-day precisions (RSD) were < or =8.92 and 10.35% and accuracy (%RE) were < or =7.33% and 9.33%, respectively. The recovery was more than 88.50% for both donepezil and internal standard by solid-phase extraction, eliminating evaporation and reconstitution steps. |
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Keywords: | human plasma donepezil multiple reaction monitoring LC‐MS/MS |
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