Trace analysis of antibacterial tylosin A, B, C and D in honey by liquid chromatography-electrospray ionization-mass spectrometry |
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Authors: | Nozal Nalda María Jesús Bernal Yagüe José Luis Gómez María Teresa Martín Jiménez Sevilla Juan José Bernal del Nozal José Higes Pascual Mariano |
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Affiliation: | Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, Spain. mjdnozal@qa.uva.es |
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Abstract: | A new LC-ESI-MS method was developed for the determination of residues of the antibacterial tylosins A, B, C and D in honey. The procedure employed an SPE on polymeric cartridges for the isolation of tylosins from diluted honey. Chromatographic separation of the tylosins was performed on a C18 column (150 x 4.60 mm2 ID, 5 microm) using a ternary gradient made of formic acid 1% in water (solvent A), methanol (solvent B) and ACN (solvent C) as mobile phase, at 30 degrees C and at a flow rate of 0.8 mL/min. Average analyte recoveries for the studied compounds ranged from 89 to 106% in replica sets of fortified honey samples. The detection limits for the four drugs studied were between 2 and 3 microg/kg. The developed method has been applied to the analysis of tylosin residues in honey from veterinarian treated beehives fed with the technical product, which contains the four compounds and is a new candidate antibiotic to treat American foulbrood disease of honey bee colonies. |
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Keywords: | Determination Honey LC‐MS Residues Tylosins |
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