| 交联酶聚集体与仿生硅化技术结合制备固定化脂肪酶 |
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| 引用本文： | 姜艳军,王旗,王温琴,周丽亚,高静.交联酶聚集体与仿生硅化技术结合制备固定化脂肪酶[J].催化学报,2012,33(5):857-862. |
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| 作者姓名： | 姜艳军 王旗 王温琴 周丽亚 高静 |
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| 作者单位： | 1. 河北工业大学化工学院,天津300130;中国科学院过程工程研究所生化工程国家重点实验室,北京100190
2. 河北工业大学化工学院,天津,300130 |
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| 基金项目： | 国家自然科学基金青年基金，河北省自然科学基金，河北省高等学校科学技术研究重点项目，河北省应用基础研究计划重点基础研究项目，生化工程国家重点实验室开放课题 |
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| 摘 要： | 将交联酶聚集体(CLEAs)与仿生硅化技术相结合,制备了交联脂肪酶Candidasp.99-125杂化生物催化剂.以京尼平为交联剂,在最佳条件下制得的脂肪酶CLEAs的酶活达771U/g,回收率达75%;保护剂聚乙烯亚胺(PEI)与Candidasp.99-125脂肪酶共沉淀制备P/CLEAs,其酶活达897U/g,回收率约88%;利用PEI的诱导作用,在P/CLEAs表面形成氧化硅涂层,制得的脂肪酶CLEAs(Coated-CLEAs)显示出良好的稳定性,特别是其抗蛋白酶水解能力、有机溶剂耐受能力、重复使用性能等方面明显提高.
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| 关 键 词： | 交联酶聚集体 仿生硅化 假丝酵母脂肪酶Candida sp. - 京尼平 固定化酶 |
Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification |
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| JIANG Yanjun
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WANG Qi
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WANG Wenqin
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ZHOU Liya
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GAO Jing.Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification[J].Chinese Journal of Catalysis,2012,33(5):857-862. |
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| Authors: | JIANG Yanjun WANG Qi WANG Wenqin ZHOU Liya GAO Jing |
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| Institution: | 1,* 1School of Chemical Engineering Hebei University of Technology,Tianjin 300130,China 2National Key Laboratory of biochemical Engineering Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China |
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| Abstract: | The hybrid biocatalyst was prepared by the combination of cross-linked enzyme aggregates(CLEAs) and biomimetic silicification,where the lipase Candida sp.99-125 was used as model enzyme.The preparation conditions of lipase-CLEAs were optimized and the resulting activity of 771 U/g was obtained.Under the optimum conditions,75% of the activity recovery was obtained.P/CLEAs was prepared by coprecipitation of lipase with polyethylene-imine(PEI),and the resulting activity was 897 U/g.The activity recovery of P/CLEAs was about 88%.The silica coating was formed on the outside surface of P/CLEAs via the induction of PEI.The resulting Coated-CLEAs showed high reusability.The stability of the Coated-CLEAs,including the resistance to trypsin and organic solvents,was also significantly improved. |
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| Keywords: | cross-linked enzyme aggregate biomimetic silicification lipase Candida sp 99-125 genipin immobilized enzyme |
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