|
|||||
|
|
Sensitive immunoassay for porcine pseudorabies antibody based on fluorescence signal amplification induced by cation exchange in CdSe nanocrystals |
|
| Authors: | XuePu Li Kun Chen Liang Huang DongLian Lu JianGong Liang HeYou Han |
| Institution: | 1. State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China |
| Abstract: | We report on a novel immunoassay for porcine pseudorabies virus (PRV) antibody that is based on fluorescence signal amplification induced by silver(I) ion exchange in CdSe nanocrystals. An antigen-antibody-secondary antibody sandwich structure was first formed from PRV, PRV antibody, and CdSe-labeled rabbit anti-pig antibody. Then, the Cd(II) ions in the CdSe labels were released by a cation exchange reaction with Ag(I). Released Cd(II) was finally quantified using the sensitive fluorescent probe Rhodamine 5 N. Due to this signal amplification, the sensitivity and linear range of the immunoassay were largely improved (compared to the traditional ELISA) in having a limit of detection as low as 1.2 ng?mL?1 of PRV antibody and a linear range from 2.44 to 312 ng?mL?1. The successful determination of PRV antibody in pig serum samples is proof for the utility of the method. A simple, rapid and sensitive method for the detection of PRV antibody through the fluorescence signal amplification caused by cation-exchange in CdSe NCs was reported. The CdSe NCs labeled rabbit anti-pig IgG was used to capture the PRV antibody. After the immunoreaction, the Cd2+ in the CdSe labels was completely replaced by the cation-exchange reaction with Ag+. Then Cd2+sensitive fluorescence indicator Rhod-5 N was added to bind with Cd2+ and caused the fluorescence signal enhance substantially. Thus a novel method for rapid and sensitive detection of porcine pseudorabies based on the fluorescence signal amplification was developed. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |