Decoding a PNA encoded peptide library by PCR: the discovery of new cell surface receptor ligands |
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Authors: | Svensen Nina Díaz-Mochón Juan José Bradley Mark |
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Affiliation: | School of Chemistry, University of Edinburgh, Joseph Black Building, Edinburgh EH9 3JJ, UK. |
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Abstract: | The ability to screen and identify new ligands for cell surface receptors has been a long-standing goal as it might allow targeting of pharmaceutically relevant receptors, such as integrins or G protein coupled receptors. Here, we present a method to amplify hits from a library of PNA-tagged peptides. To this end, human cells, overexpressing either integrins or the CCR6 receptor, were treated with a 10,000 member PNA-encoded peptide library. Extraction of the PNA tags from the surface of the cells was followed by a PNA-tag to DNA translation and amplification enabling decoding of the tags via microarray hybridization. This approach to ligand discovery facilitates screening for differences in surface-receptor ligands and/or receptor expression between different cell types, and opens up a practical approach to PNA-tag amplification. |
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