Effective PCR detection of animal species in highly processed animal byproducts and compound feeds |
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Authors: | Olivier Fumière Marc Dubois Vincent Baeten Christoph von Holst Gilbert Berben |
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Institution: | (1) Département Qualité des Productions Agricoles, Centre Wallon de Recherches agronomiques (CRA-W), 24 Chaussée de Namur, 5030 Gembloux, Belgium;(2) European Commission, Directorate-General Joint Research Centre, Institute for Reference Materials and Measurments (JRC-IRMM), 111 Retieseweg, 2440 Geel, Belgium |
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Abstract: | In this paper we present a polymerase chain reaction (PCR)-based method for detecting meat and bone meal (MBM) in compound
feedingstuffs. By choosing adequate DNA targets from an appropriate localisation in the genome, the real-time PCR method developed
here proved to be robust to severe heat treatment of the MBM, showing high sensitivity in the detection of MBM. The method
developed here permits the specific detection of processed pig and cattle materials treated at 134 °C in various feed matrices
down to a limit of detection of about 0.1%. This technique has also been successfully applied to well-characterised MBM samples
heated to as high as 141 °C, as well as to various blind feed samples with very low MBM contents. Finally, the method also
passed several official European ring trials. |
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Keywords: | Feed Meat and bone meal (MBM) BSE Polymerase chain reaction Real time PCR Mitochondrial DNA Species identification Pig Cattle |
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