Fixation of amino acids and oligopeptides by freeze-drying in polyacrylamide gel electrophoresis |
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Authors: | H Nishizawa K Suzuki Y Abe |
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Institution: | Kyoritsu College of Pharmacy, Tokyo, Japan. |
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Abstract: | Following polyacrylamide gel electrophoresis in an acetic/formic acid buffer, pH 2.0, in fabric reinforced gels, amino acids and oligopeptides could successfully be fixed in the gel by freeze-drying. Lyophilization of the fabric reinforced polyacrylamide gel after electrophoresis resulted in a dry film which absorbed ninhydrin solution quickly and uniformly, thus improving the detection limit for amino acids and oligopeptides with molecular weights ranging from 189-1045. Most amino acids were detected with a sensitivity of 0.1-0.25 microgram and for oligopeptides the detection limit was found to be 0.5-5 microgram. |
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