微乳液相色谱法测定人血浆中丙泊酚浓度

建立了基于微乳液相色谱(MELC)的人血浆中丙泊酚浓度的测定方法。采用Hypersil BDS C18色谱柱分离,并考察了微乳流动相中各组分对溶质洗脱的影响。优化的色谱条件: 以0.5%醋酸（含有3.0%十二烷基硫酸钠（SDS）,0.8%正庚烷,6.0%正丁醇）微乳为流动相,流速为1.0 mL/min,荧光检测器激发波长(λex)为274 nm、发射波长(λem)为312 nm,柱温为室温。人血浆样品用流动相稀释并离心后,直接进样分析。丙泊酚在0.25～10 μg/mL质量浓度范围内呈良好的线性关系,方法的回收率为(98.2±1.9)%～(104.6±2.2)%;日内测定峰面积的相对标准偏差(RSD)为1.42%～2.43%,日间测定峰面积的RSD为2.75%～4.79%。该方法简便可行、重复性好,可用于人血浆中丙泊酚浓度的测定。

Determination of propofol in human plasma by microemulsion liquid chromatography

A microemulsion liquid chromatographic (MELC) method was developed for the determination of propofol in human plasma. The assay was performed on a Hypersil BDS C18 column, and the effects of each component in the microemulsion mobile phase on the elution were investigated. The optimized microemulsion mobile phase consisted of 0.5% acetic acid containing 3.0% sodium dodecylsulfate, 0.8% n-heptane, 6.0% n-butanol. The flow rate was 1.0 mL/min, and the separation was operated at ambient temperature. The fluorescence excitation and emission wavelengths were 274 nm and 312 nm, respectively. The plasma samples were diluted with the mobile phase and centrifuged before injection. The calibration curve of propofol was linear within the range of 0.25 - 10 microg/mL. The method recoveries were (98.2 +/- 1.9)% - (104.6 +/- 2.2)%. The intra-day relative standard deviations (RSDs) of peak area were 1.42% -2.43%, and the inter-day RSDs were 2.75% - 4.79%. The method is simple and reproducible. It can be used for the determination of propofol in human plasma.