火焰原子吸收法鼠肺组织总钙

本经十二烷箕硫酸钠，十二烷基二甲基苄基溴化铵和壬基酚基聚氧乙烯醚复配而成的混合微乳液体系对鼠肺组织进行浸取前处理，用火焰原子吸收法测定了其中的钙含量。用混合微乳淮法与溶液法进行了一系列对照试验，结果表明：在灵敏度、精密度、选择性、工作曲线线性方面均优于后。检出限为１．９２ｎｇ·ｃｍ＾－３，相对标准偏差为１．９２％（ｎ＝１０），工作曲线线性范围０￣２０μｇ·ｃｍ＾－３，线性回归系数ｒ＝０．９９９

Determination of total Ca in the lung tissue of rats by FAAS]

Determination of total Ca in the Lung Tissue of Rats by FAAS was described, with a mixed microemulsion consisting of sodium dodecyl sulfate, N-dodecyl, N-dimethyl benzol ammonium bromide and nonyl phenol polyethyeneoxy ether. Comparing the result of the microemulsion FAAS with that of solution FAAS showed that the former was better than the latter in sensitivity, precision, selectivity and linearity of the calibration curve, with a detection limit of 1.92 microg x cm(-3), relative standard deviation of 1.92% (n = 10) and linear regressive coefficient of 0.9998 of the calibration curve over 0-20 microg x cm(-3) calcium. The method was applied to determine calcium amount in the lung tissue of rats, and the results were in accordance with those odtained by the conventional solution FAAS, in which a sample was reduced to ashes at a high temperature.