Synthesis and immunological study of a wall teichoic acid-based vaccine against E. faecium U0317 |
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Authors: | Zhifang Zhou Wenzhang Ding Chen Li |
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Institution: | 1. Key Laboratory of Carbohydrate Chemistry &2. Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China |
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Abstract: | ABSTRACTA repeat unit of cell wall teichoic acids (WTA) isolated from E. faecium U0317 was chemically synthesized efficiently by a stepwise strategy. It was derivatized with a 5-aminopentanyl linker to facilitate conjugation with carrier proteins KLH and HSA. Immunological studies of the KLH conjugate 1 demonstrated that it could provoke robust immune responses and high titers of IgG antibodies, which could successfully recognize the synthesized WTA repeat unit 3. This result suggested that synthetic glycoconjugate 1 could be a promising vaccine candidate against E. faecium for further studies. |
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Keywords: | E feacium wall teichoic acid vaccine glycoconjugate |
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