Evaluation of the effect of Ramadan fasting on fat-soluble antioxidants and markers of oxidative stress in healthy Pakistani subjects |
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Authors: | Abad Khan Zafar Iqbal |
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Affiliation: | 1. Department of Pharmacy, University of Swabi, Peshawar, Pakistandrabadkhan@uoswabi.edu.pk;3. Department of Pharmacy, University of Peshawar, Peshawar, Pakistan |
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Abstract: | The aim of this study is to evaluate the effect of Ramadan fasting on the fat-soluble antioxidants [all-trans-retinol (vitamin A) and α-tocopherol (vitamin E)] in healthy Pakistani subjects. Thirty (30) healthy male volunteers (aged 30–50 years) with Ramadan fasting have participated in the study. Blood sampling from these subjects was conducted 1 day before and on 15th and 28th days of Ramadan. The serum levels of vitamin A and vitamin E were measured using liquid chromatography linked with UV–visible (HPLC–UV). The comparison between the samples taken at different times was made by Minitab software applying two samples and paired t-test at (p?0.05) as significant. No significant changes have been found in the levels of all-trans-retinol before and during Ramadan values. The levels of α-tocopherol on 28th Ramadan have been decreased significantly when compared with its values before Ramadan (p?0.0001). It is concluded that there is nonsignificant change in the levels of vitamin A during the month of Ramadan while the levels of α-tocopherol have been decreased significantly on the 28th day of Ramadan. It is therefore suggested that the fasting of Ramadan has effect on the levels of α-tocopherol and food-based interventions might be necessary to modify the diet during Ramadan. |
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Keywords: | Antioxidants fasting healthy HPLC–UV samples |
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