| Kalman滤波荧光光度法同时测定酪氨酸、色氨酸和苯丙氨酸 |
| |
| 引用本文： | 张晶玉,杨金夫,任英,高丽云.Kalman滤波荧光光度法同时测定酪氨酸、色氨酸和苯丙氨酸[J].光谱学与光谱分析,1994(5). |
| |
| 作者姓名： | 张晶玉 杨金夫 任英 高丽云 |
| |
| 作者单位： | 中国科学院长春应用化学研究所，长春冶金地质专科学校 |
| |
| 基金项目： | 中国科学院长春分院资助 |
| |
| 摘 要： | 苯丙氨酸的荧光峰完全包含在酪氨酸荧光峰之中，酪氨酸和色氨酸的荧光峰也严重重叠。用卡尔曼滤波法解析该体系给出了令人满意的分析结果。回收率为９４．０～１０５％，相对标准偏差不大于３．６％（个别一点除外）。将试剂空白作为一个组分计算，从而免去了试剂空白扣不准给分析结果带来的影响。利用新息序列的白噪音特性来判断结果的可靠性。
|
| 关 键 词： | 荧光光度法，卡尔曼滤波法，氨基酸 |
SIMULTANEOUS DETERMINATION OF TRYPTOPHEN,PHENYLALANINE AND TYROSINE BY KALMAN FILTERING IN FLUOROSPECTROPHOTOMETRY |
| |
| ZHANG Jingyu,YANG Jinfu,REN Ying and GAO LiyunChangchun Institute of Applied Chemistry,Academia Sinica,Changchun.SIMULTANEOUS DETERMINATION OF TRYPTOPHEN,PHENYLALANINE AND TYROSINE BY KALMAN FILTERING IN FLUOROSPECTROPHOTOMETRY[J].Spectroscopy and Spectral Analysis,1994(5). |
| |
| Authors: | ZHANG Jingyu YANG Jinfu REN Ying and GAO LiyunChangchun Institute of Applied Chemistry Academia Sinica Changchun |
| |
| Institution: | ZHANG Jingyu,YANG Jinfu,REN Ying and GAO LiyunChangchun Institute of Applied Chemistry,Academia Sinica,130022Changchun |
| |
| Abstract: | Simultaneous fluorospectrophotometric deterrnination of tryptophen,tyrosine and phenylalanine was investigated with the use ofa Kalman filtering technique for the resolution of overlapping emission spectra of these components. For all the synthetic samples with only one exception,the recoverles are 94. 0% to 105%,and the relative standard deviations are less than 3. 6%.The whiteness of the innovation sequence for an optimal fliter was explored to be the criterion for evaluating the rellability ofanalytical results.The blank was treated as a componentand all spectral scans thereforedld notrequire the subtraction for blank. |
| |
| Keywords: | Fluorospectrophotometry Kalman filtering Amino acid |
| 本文献已被 CNKI 等数据库收录！ |
