| 树脂吸附法制备固定化乳糖酶及其操作稳定性的研究 |
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| 引用本文： | 俞宏峰,孙志浩,郑璞,张伟,范云六. 树脂吸附法制备固定化乳糖酶及其操作稳定性的研究[J]. 离子交换与吸附, 2006, 22(5): 423-428 |
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| 作者姓名： | 俞宏峰 孙志浩 郑璞 张伟 范云六 |
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| 作者单位： | 江南大学生物工程学院,工业生物技术教育部重点实验室,无锡,214036;中国农业科学院生物技术研究所,北京,100081 |
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| 基金项目： | 国家自然科学基金（20476039）、国家“973”（2003cB716008）和长江学者和创新团队发展计划资助（IRT0532） |
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| 摘 要： | 利用树脂吸附并结合包埋与交联的方法进行微生物胞外乳糖酶的固定化。固定化乳糖酶体现了良好的操作稳定性。以5%乳糖溶液为底物,分批反应11次后,乳糖水解率仍保持85%以上;在填充床反应器中进行连续水解乳糖实验,反应62天后,乳糖水解率保持在80%。
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| 关 键 词： | 乳糖酶 固定化 树脂 吸附 操作稳定性 |
| 文章编号： | 1001-5493(2006)05-0423-06 |
| 收稿时间： | 2005-11-16 |
| 修稿时间： | 2005-11-16 |
PREPARATION OF THE IMMOBILIZED LACTASE BY ABSORPTION WITH RESIN AND ITS OPERATIONAL STABILITY |
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| YU Hongfeng,SUN Zhihao,ZHENG Pu,ZHANG Wei,FAN Yunliu. PREPARATION OF THE IMMOBILIZED LACTASE BY ABSORPTION WITH RESIN AND ITS OPERATIONAL STABILITY[J]. Ion Exchange and Adsorption, 2006, 22(5): 423-428 |
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| Authors: | YU Hongfeng SUN Zhihao ZHENG Pu ZHANG Wei FAN Yunliu |
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| Affiliation: | 1. School of Biotechnology Southern Yangtze University, Key Laboratory of lndustry Biotechnology, Ministry of Education, Wuxi 214036, China; 2. Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China |
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| Abstract: | The immobilized lactase was prepared by resin absorption, entrap and crosslink. It exhibited excellent operational stability. With 5% lactose as the substrate, the lactose hydrolysis was maintained at 85% after 11 cycles; the lactose hydrolysis was kept at 80% when the immobilized lactase was applied in the packed-bed reactor after 62 days. |
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| Keywords: | Lactase Immobilization Resin Absorption Operational stability. |
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