Bio-electrocatalysis of NADH and ethanol based on graphene sheets modified electrodes |
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Authors: | Guo Kai Qian Kun Zhang Song Kong Jilie Yu Chengzhong Liu Baohong |
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Institution: | a Department of Chemistry and Key Lab of Molecular Engineering of Polymers of Chinese Ministry of Education, Fudan University, Shanghai 200433, PR China b ARC Centre of Excellence for Functional Nanomaterials and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia |
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Abstract: | Characterization and application of graphene sheets modified glassy carbon electrodes (graphene/GC) have been presented for the electrochemical bio-sensing. A probe molecule, potassium ferricyanide is employed to study the electrochemical response at the graphene/GC electrode, which shows better electron transfer than graphite modified (graphite/GC) and bare glassy carbon (GC) electrodes. Based on the highly enhanced electrochemical activity of NADH, alcohol dehydrogenase (ADH) is immobilized on the graphene modified electrode and displays a more desirable analytical performance in the detection of ethanol, compared with graphite/GC or GC based bio-electrodes. It also exhibits good performance of ethanol detection in the real samples. From the results of electrochemical investigation, graphene sheets with a favorable electrochemical activity could be an advanced carbon electrode materials for the design of electrochemical sensors and biosensors. |
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Keywords: | Graphene Alcohol dehydrogenase NADH Ethanol biosensor |
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