| Polarographic immunoassay coupled with catalysis of non-radioactive multiple iodine label |
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| 作者姓名： | 宋俊峰 白亚丽 过玮 贾晓琳 |
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| 作者单位： | Institute of Electroanalytical Chemistry，Northwest University，Xi an 710069，China，Institute of Electroanalytical Chemistry，Northwest University，Xi an 710069，China，Institute of Electroanalytical Chemistry，Northwest University，Xi an 710069，China，Aerospace Central Hospital，Heijing 100039，China |
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| 摘 要： | A now polarographic immunoassay was developed In this assay,human serum albumin (HSA) as the model antigen was covalently labeled with organic compound erythrosin B(EB) containing four non-radioactive iodides through Ⅰ step chemical reaction The labeling procedure is simple and the conditions needed are moderate.The molar labeling ratio of KB HSA was 12 Ⅰ The content of iodine in the conjugate obtained by the proposed procedure is ninth higher than that by the other existing methods.A heterogeneous competitive immunoassay was established by compling the catalysis of the conjugate to substrate As(Ⅲ)-Ce(Ⅳ) reaction with the linear-sweep polarographic detec-tion of As(Ⅲ) amount HSA can be determined in the HSA concentration range from 1 to 200μg/mL,with the de-tection hum of 0 66μg/ml.
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| 收稿时间： | 21 November 1996 |
Polarographic immunoassay coupled with catalysis of non-radioactive multiple iodine label |
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| Junfeng Song,Yali Bai,Wei Guo,Xiaolin Jia.Polarographic immunoassay coupled with catalysis of non-radioactive multiple iodine label[J].Science in China(Chemistry),1997,40(5):457-463. |
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| Authors: | Junfeng Song Yali Bai Wei Guo Xiaolin Jia |
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| Institution: | (1) Northwest University, Institute of Electroanalytical Chemistry, 710069 Xi’an, China;(2) Aerospace Central Hospital, 100039 Beijing, China |
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| Abstract: | A new polarographic immunoassay was developed. In this assay, human serum albumin (HSA) as the model antigen was covalently
labeled with organic compound erythrosin B (EB) containing four non-radioactive iodides through 1-step chemical reaction.
The labeling procedure is simple and the conditions needed are moderate. The molar laheling ratlo of EB: HSA was 12: 1. The
content of iodine in the conjugate obtained by the proposed procedure is much higher than that by the other existing methods.
A heterogeneous competitive immunoassay was established by coupling the catalysis of the conjugate to substrate As (III) -Ce
(IV) reaction with the linear-sweep polarographic detection of As (III) amount. HSA can be determined in the HSA concentration
range from 1 to 200 μg/mL, with the detection limit of 0. 66,μg/mL.
Project supported by the National Natural Science Foundation of China |
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| Keywords: | HSA erythrosin B immunoassay catalysis linear-sweep polarography |
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