记忆缺失性贝类毒素的主要成分——软骨藻酸的毛细管电泳分析

 通过萃取、离子交换等技术 ,建立了毛细管电泳 /紫外检测法分析海洋赤潮生物毒素的重要种类之一、记忆缺失性贝类毒素的主要成分软骨藻酸的方法。结果表明 :软骨藻酸在 0 2mg/L～ 5 0mg/L时具有良好的线性关系 ,相关系数r =0 9990 ;方法检出限为 0 0 6 3mg/L(S/N >3)。在 3个添加水平上进行加标回收试验 (n =6 ) ,平均回收率分别为 97 2 4% ,96 92 %和 97 5 5 % ,RSD分别为 2 .74% ,2 .5 9%和 1.95 %。利用该方法对 5种经济贝类样品进行了测定。该方法简单、灵敏、高效、成本低 ,对软骨藻酸的检测和监控具有重要意义。

Capillary Electrophoretic Analysis of Amnesic Shellfish Toxin-Domoic Acid

Domoic acid, a main component of amnesic shellfish toxin (one of the four sorts of marine red tide bio-toxins) was determined by capillary electrophoresis with UV detection. The shellfish samples were prepared by solvent extraction, cleaned-up with a strong anion-exchange cartridge (SAX: Part No. 1210-2044, Lot No. 182639, Varian) and a cation-exchange cartridge (SCX: Part No. 1211-3039, Lot No. 171069, Varian). The quantitative analysis was performed with external standard under the optimum conditions of capillary electrophoretic analysis. The calibration curve of domoic acid showed good linearity in the range of 0.2 mg/L-50 mg/L with r = 0.9990, and the detection limit was 0.063 mg/L(S/N > 3). Spiked with standard at three levels (5.00 micrograms/g, 10.00 micrograms/g and 20 micrograms/g), the samples had the recoveries of 97.24%, 96.92% and 97.55%, and the RSDs of 2.74%, 2.59% and 1.95%, respectively. Five kinds of shellfish samples normally consumed in Dalian sea area were collected and quantified. The method has the advantages of being simple, convenient, sensitive and low cost. Therefore it can be used as one of the routine monitoring methods for amnesic shellfish toxin.