Isolation and Extraction of Total Flavonoids from Epimedium Koreanum Nakai by Supercritical Fluid Extraction |
| |
Authors: | LIU Chun-ming ZHAO Xue-liang LIU Zhi-qiang XING Jun-peng |
| |
Institution: | 1. Laboratory of New Drug Research, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China;
2. Department of Chemistry, Changchun Teachers College, Changchun 130032, P. R. China;
3. China-Japan Union Hospital, Jilin University, Changchun 130032, P. R. China |
| |
Abstract: | In the present paper is reported the method for the isolation and extraction of total flavonoids of Epimedium Koreanum Nakai by means of supercritical fluid extraction(SFE). By examining pressure, temperature, amounts of modifier and extraction time, the optimized condition of SFE is confirmed as 30 MPa and 60 ℃, with 70% ethanol as the modifier. The samples were statically extracted for 30 min, followed by dynamic extraction for 120 min at a flow rate of 6 mL/min. The quantitative analysis of total flavonoids was performed by UV-Vis spectrophotometry. Compared with the conventional method, the SFE method is more efficient, more rapid and more friendly environmentally. |
| |
Keywords: | Supercritical fluid extraction(SFE) Flavonoids Epimedium Koreanum Naka |
本文献已被 CNKI 维普 万方数据 等数据库收录! |
| 点击此处可从《高等学校化学研究》浏览原始摘要信息 |
| 点击此处可从《高等学校化学研究》下载免费的PDF全文 |
|