Quantitative LC-ESI-MS/MS metabolic profiling method for fatty acids and lipophilic metabolites in fermentation broths from β-lactam antibiotics production |
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Authors: | Simone Schiesel Michael Lämmerhofer Wolfgang Lindner |
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Institution: | (1) Christian-Doppler Laboratory for Molecular Recognition Materials, Department of Analytical Chemistry and Food Chemistry, University of Vienna, Waehringer Strasse 38, 1090 Vienna, Austria; |
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Abstract: | In the present paper, we report on the development of a straightforward reversed-phase liquid chromatography–electrospray
ionization–tandem mass spectrometry method for the determination of the most abundant fatty acids; α-tocopherol and cephalosporin
P1 in fermentation broths. Using this method, fatty acids could be successfully determined in extracts of fermentation broths
from penicillin and cephalosporin production without prior derivatization. Matrix effects were investigated in detail, and
various kinds of calibrations (i.e., by use of neat standard solutions as well as by matrix-matched calibration employing
standard addition each with and without internal standards) were comparatively assessed. The optimized and validated method
was employed for the analysis of extracts of fermentation broths and nutrition media. |
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