Image edge-enhancement in optical microscopy with a phase mismatched spiral phase plate |
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Authors: | Shibiao Wei Jing Bu Siwei Zhu Xiaocong Yuan Institute of Modern Optics Key Laboratory of Optoelectronic Information Science Technology Ministry of Education of China Nankai University Tianjin China Nankai University Affiliated Hospital Tianjin China |
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Affiliation: | Shibiao Wei 1,Jing Bu 1,Siwei Zhu 2,and Xiaocong Yuan 1 1Institute of Modern Optics,Key Laboratory of Optoelectronic Information Science and Technology,Ministry of Education of China,Nankai University,Tianjin 300071,China 2Nankai University Affiliated Hospital,Tianjin 300121,China |
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Abstract: | We present a spiral phase filtering system with a large tolerance for edge enhancement of both phase and amplitude objects in optical microscopy.The method is based on a Fourier 4-f spatial filtering system.A phase mismatched spiral phase plate (SPP) fabricated by electron beam lithography is employed as the radial Hilbert transform for image edge enhancement.Compared with holography,SPP is simple,economical,reliable,and easy to integrate. |
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