Impact of selective excitation on carbon longitudinal relaxation: Towards fast solid-state NMR techniques |
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Authors: | Mathilde Giffard Michel Bardet Beate Bersch Jacques Covs Sabine Hediger |
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Institution: | aLaboratoire de Chimie Inorganique et Biologique (UMR-E3 CEA/UJF, FRE 3200 CEA/CNRS), INAC, CEA, F-38054 Grenoble, France;bInstitut de Biologie Structurale (UMR 5075 CEA/CNRS/UJF), 38027 Grenoble, France |
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Abstract: | The effect of selective pulses on the apparent carbon longitudinal relaxation is investigated in three fully 13C-labeled systems, histidine as a model system and two proteins MerP and YajG. It is shown that the longitudinal relaxation of a selectively excited carbon spin is greatly enhanced, mainly because of fast spin-diffusion. This relaxation enhancement allows reducing the time necessary for polarization recovery between two experiments. This effect can be exploited either to improve the sensitivity of NMR experiments or to reduce the experimental time. Using selective carbon excitation combined with fast pulsing on fully 13C-labeled proteins, a sensitivity improvement of 20–45% over standard cross-polarization methods is predicted from the measured relaxation times. |
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Keywords: | Solid-state NMR Proteins Longitudinal relaxation Fast NMR Selective pulses Biomolecules Spin-diffusion Sensitivity enhancement |
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