二茂铁衍生物电子传递剂键合的溶胶-凝胶葡萄糖生物传感器

The sol-gel derived glucose biosensor was developed, and the sol-gel membrane was organically modified by N-（3-triethoxysilylpropyl）-ferrocenylmethylamine （FcSi） as sol-gel precursor to make electrochemical biosensor. The structure of biosensor was sol-gel/FcSi＋GOx/GC type （glucose oxidase, GOx）. The ferrocene mediator was chemically immobilized to the silane network, and GOx was entrapped to the sol-gel glass network. Therefore, these structures prevented mediator leakage and retained the enzyme activity. Additionally, pH of electrolyte, temperature effects, and interference of positive substances with biosensor were investigated. And the electrochemical performance of biosensor was studied by amperometry. The results indicated that the linear range, detection limit. and response slope of biosensor was 2.00×10^-4-1.57×10^-3 mol·L^-1, 2.0×10^-4 mol·L^-1 and 5.06×10^5 nA·mol^- 1·L, respectively.

Ferrocene Derivative Mediator Bonded Sol-gel Membrane Glucose Biosensor

The sol‐gel derived glucose biosensor was developed, and the sol‐gel membrane was organically modified by N‐(3‐triethoxysilylpropyl)‐ferrocenylmethylamine (FcSi) as sol‐gel precursor to make electrochemical biosensor. The structure of biosensor was sol‐gel/FcSi+GOx/GC type (glucose oxidase, GOx). The ferrocene mediator was chemically immobilized to the silane network, and GOx was entrapped to the sol‐gel glass network. Therefore, these structures prevented mediator leakage and retained the enzyme activity. Additionally, pH of electrolyte, temperature effects, and interference of positive substances with biosensor were investigated. And the electrochemical performance of biosensor was studied by amperometry. The results indicated that the linear range, detection limit, and response slope of biosensor was 2.00×10^?4–1.57×10^?3 mol·L^?1, 2.0×10^?4 mol·L^?1 and 5.06×10⁵ nA·mol^?1·L, respectively.