A quantitative approach to the analysis of supermicron dispersions by field-flow fractionation with UV-vis detectors. The application of an absolute method |
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Authors: | P Reschiglian D Melucci G Torsi |
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Institution: | (1) Department of Chemistry “G. Ciamician”, University of Bologna, V. Selmi, 2, 40126 Bologna, Italy |
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Abstract: | Summary Quantitative analysis in field-flow fractionation is becoming a necessary requirement for routine applications, instrumental
optimization and scale-up to preparative separations. The use of detection systems which show complex dependence on sample
characteristics (i.e. UV spectrometry) has hindered the application of quantitative methods of analysis in field-flow fractionation.
A standardless model, shown valid in flow-through, homogeneous systems, is applied here to a heterogeneous system (dispersed
supermicron particles) in field-flow fractionation by single peak area measurements. Absolute analysis in the fractionation
of spherical silica particles for high-performance liquid chromatography column packing by gravitational field-flow fractionation
with UV-Vis detectors is presented. It has been shown that for such samples extinction coefficients are independent of sample
concentration and are determined by the size and density of the particles. The accuracy of such an approach to absolute analysis
is discussed.
In memory of J. C. Giddings Presented at FFF'95-Fifth International Symposium on Field-Flow Fractionation, Park City, UT,
USA, July 10–12 1995. |
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Keywords: | Field-flow fractionation Particulate matter analysis Turbidimetric detection Analysis without standards |
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