Influence of injection parameters on column performance in nanoscale high-performance liquid chromatography |
| |
Authors: | S. Héron A. Tchapla J. -P. Chervet |
| |
Affiliation: | (1) LETIAM-IUT d’Orsay, Plateau de moulon, 91400 Orsay, France;(2) LC Packings, Baarsjesweg 154, 1057 HM Amsterdam, The Netherlands |
| |
Abstract: | Summary The influence of the injection volume and the sample solvent on column efficiency has been evaluated in packed nano liquid chromatography using columns 150μ i.d. Evaluation of column performance was by means of reduced plate height (h) versus reduced velocity (v) for four polyaromatic hydrocarbon test compounds (PAHs). When compounds are dissolved in a weak solvent (such as MeCN: H2O, 30∶70), and whatever the injection volume −60 or 200 nL-a gain in efficiency can be observed due to the well-known on-column focusing phenomenon, but keeping constant solute retention factors. Under optimized conditions (flow rate: 150 nLmin−1, solvent sample MeCN: H2O, 30∶70, injection volume 200 nL), a reduced plate height of 1.83 has been obtained for a 15 μm C18 packing corresponding to 36000 plates m−1, which illustrates the absence of any extracolumn band broadening under nano LC conditions. |
| |
Keywords: | Column liquid chromatography Micropacked columns Injection methods Column efficiency vs sample volume |
本文献已被 SpringerLink 等数据库收录! |
|