Synthesis of f luorescein-labeled oligonucleotides bearing a tag in position 2′ of modified adenosine and arabinoadenosine |
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| Authors: | S. V. Vasileva E. E. Krasnousova A. A. Donina T. V. Abramova L. G. Zhdanova S. P. Kovalenko V. N. Silnikov |
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| Affiliation: | (1) Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 8 prosp. Akad. Lavrent’eva, 630090 Novosibirsk, Russian Federation;(2) Novosibirsk State University, 2 ul. Pirogova, 630090 Novosibirsk, Russian Federation;(3) The OJSC Medigen Laboratory, 2 ul. Timakova, 630117 Novosibirsk, Russian Federation |
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| Abstract: | Oligonucleotide conjugates containing fluorescein residues in the sugar-phosphate back-bone were synthesized by the standard solid-phase phosphoramidite method using phosphor-amidites of 9-[2-deoxy-5-O-(4,4′-dimethoxytrityl)-2-methoxalylamino-β-D-ribofuranosyl]-and 9-[2-deoxy-5-O-(4,4′-dimethoxytrityl)-2-methoxalylamino-β-D-arabinofuranosyl]-N 6-benzoyladenine. The relative efficiency of the oligonucleotide synthesis with modified phosphoramidites was estimated. Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 9, pp. 1618–1624, September, 2006. |
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| Keywords: | 2′ -modified nucleosides reactive linker group oligonucleotide synthesis |
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