Primer extension reactions as a tool to uncover folding motifs within complex G-rich sequences: analysis of the human KRAS NHE |
| |
| Authors: | Paramasivam Manikandan Cogoi Susanna Xodo Luigi E |
| |
| Affiliation: | Department of Medical and Biological Science, University of Udine, 33100 Udine, Italy. |
| |
| Abstract: | We employed primer extension reactions to uncover folding motifs in a nuclease hypersensitive element (NHE) with a complex guanine pattern, located in the human KRAS promoter. We also identified and characterized a new G-rich motif of 21 nt capable of forming a parallel G-quadruplex that is disrupted by protein UP1. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
