Cytopathology of the trachea of Bombyx mori (Lepidoptera: Bombycidae) to Bombyx mori nucleopolyhedrovirus |
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| Institution: | 1. Department of Entomology, Faculty of Science, Cairo University, P. O. Box 12613, Giza, Egypt;2. Key Lab for Biological Control of the Ministry of Agriculture, Department of Entomology, China Agricultural University, Beijing, 100193, PR China;3. Unité DGIMI UMR INRA-UM 1333, Université Montpellier, Place Eugène Bataillon, Montpellier, France;1. College of Computer and Information Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2. College of Physics and Information Engineering, FuZhou University, Fuzhou 350002, China;3. Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;1. School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China;2. National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China;1. Pólo Barreto, Universidade Federal do Rio de Janeiro, Campus Macaé, Avenida São José do Barreto, 764, São José do Barreto, Macaé, Rio de Janeiro CEP: 27910-970, Brazil;2. Laboratório de Biologia de Helmintos Otto Wucherer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco G, Ilha do Fundão, 21949-900 Rio de Janeiro, RJ, Brazil;3. Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Instituto Nacional de Biologia Estrutural e Bioimagem-INBEB, and Centro Nacional de Biologia Estrutural e Bioimagem-CENABIO, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco G, Ilha do Fundão, 21949-900 Rio de Janeiro, RJ, Brazil;4. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres e Reservatórios, Instituto Oswaldo Cruz, Av. Brasil 4365, 20010-900 Rio de Janeiro, RJ, Brazil |
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| Abstract: | Bombyx mori is a holometabolous insect found only in germplasm banks and morphological data related of resistance and susceptibility to diseases is important when selecting hybrids for commercial and scientific interest. This study analyzed the cytopathology of B. mori trachea to BmNPV, isolated geographically in Paraná state, Brazil. Fifth instar larvae were divided into two groups, control and inoculated; the viral suspension used was 2.4 × 107 polyhedral occlusion bodies/mL. From the second to the ninth day post-inoculation, segments of silkworm organs, containing the trachea, were processed for transmission electron microscopy. Analyses of fresh hemolymph were also performed to verify the susceptibility of the hemocytes. Signs of infection were initially detected in the hemocytes and in the tracheal cells on the second and fourth post-inoculation days, respectively. The cytopathology of the trachea showed all stages of the viral cycle, which was the same as in other tissues. Virions were detected in the basal lamina, which showed structural disorganization. So, the infection time of the hemocytes prior to trachea and the presence of virus in basal lamina, suggests that the trachea was a secondary target, infected by budded virus coming from the hemolymph. |
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| Keywords: | Baculoviridae Lepidoptera Tracheal cells |
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