| 草药物与人血白蛋白相互作用的分光光度法研究 |
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| 引用本文： | 刘永和,刘改云,黄海燕.草药物与人血白蛋白相互作用的分光光度法研究[J].邵阳学院学报(自然科学版),2010,7(1):53-56. |
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| 作者姓名： | 刘永和 刘改云 黄海燕 |
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| 作者单位： | 1. 邵阳学院,生物与化学工程系,湖南,邵阳,422000
2. 邵阳学院,图书馆,湖南,邵阳,422000 |
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| 基金项目： | 湖南省教育厅科研基金 |
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| 摘 要： | 以曙红Y为光谱探针,采用分光光度法探讨抗癌药物足叶乙甙对人血白蛋白中曙红Y的竞争结合规律,研究足叶乙甙与人血白蛋白的相互作用及结合.结果表明,曙红Y与人血白蛋白以静电作用力为主进行结合,形成电子转移配合物.曙红Y与人血白蛋白结合后,药物足叶乙甙的加入对这种结合产生破坏,争夺并占据人血白蛋白的结合点位,使曙红Y从结合态变成游离态,同时生成新的更为稳定的结合体—人血白蛋白与药物足叶乙甙复合物,其结合比约为7.93molVp16/molHSA.由此说明:足叶乙甙不仅与人血白蛋白存在有效的结合点位,而且具有较好的结合强度和结合容量.这些既是足叶乙甙在人体内贮存和运输的条件,也是其产生抗癌活性的药理基础.
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| 关 键 词： | 人血白蛋白 足叶乙甙 曙红Y 分光光度法 |
Study on Spectrophotometry of the Interaction Between Drug and Human Serum Albumin |
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| LIU Yong-he,LIU Gai-yun,HUANG Hai-yan.Study on Spectrophotometry of the Interaction Between Drug and Human Serum Albumin[J].Journal of Shaoyang University:Science and Technology,2010,7(1):53-56. |
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| Authors: | LIU Yong-he LIU Gai-yun HUANG Hai-yan |
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| Institution: | LIU Yong-he1,LIU Gai-yun2,HUANG,Hai-yan1 (1.Department of Biological , Chemical Engineering Shaoyang College,Shaoyang,Hunan 422000,2. Library shao yang college,Shao Yang,Hunan 422000) |
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| Abstract: | This study attempts to investigate the antineoplastic (Vp16)'s competitive binding principle toward Eosin Y in Human Serum Albumin (HSA),and explore the interaction between Etoposide and HAS as well as its binding ratio,by using Eosin Y as the spectroscopic probe. The result shows that Eosin Y and HSA were combined in the form of electrostatic interactions,to form the electron transfer complex. After the combination of Eosin Y and Human Serum Albumin,Drug Etoposide will destroy the combination to take up th... |
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| Keywords: | human serum albumin etoposide eosin Y spectrophotometry |
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